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Abstract

Intestinal parasitic infections (IPIs) continue as a substantial public health issue in evolving nations, with pediatric population’s presence particularly susceptible. This study examines the prevalence of Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum among children in Kut, Iraq, using both conventional microscopy and Multiplex PCR methodologies. A total of 257 stool specimens were procured from children aged 0 to 14 years crossways various hospitals and healthcare institutions. The specimens underwent an initial macroscopic and microscopic evaluation, subsequently followed by DNA extraction and examination by Multiplex PCR to supplement the detection capabilities for parasitic pathogens. The collective infection rate was determined to be 42.41%, with a larger prevalence experiential in females (49.24%) when compared with males (35.20%). Age-group analysis indicated that children aged 12 to 14 years showed the highest infection rate (60.00%), while the lowest prevalence was renowned in children under three years of age (37.50%). A proportional analysis between urban and rural environments revealed a heightened infection prevalence in rural locales (55.17% vs. 41.18%). The diagnostic efficiency of microscopy and Multiplex PCR confirmed considerable concordance, with Pearson correlation coefficients above 0.99 for all three parasites, so representative a healthy contract between the two diagnostic approaches. Nevertheless, Multiplex PCR shown a slightly superior detection rate for Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum. These results emphasize the authoritative for the addition of molecular diagnostic practices into standard clinical repetition to improve the sensitivity and accurateness of IPI detection, particularly within resource-constrained environments.

Keywords

Pediatric Patient Microscopy Cryptosporidium parvum Multiplex PCR Chi-Square

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Abed, R. J. (2024). THE PREVALENCE OF INTESTINAL PARASITIC INFECTIONS AMONG PEDIATRIC PATIENTS IN KUT, IRAQ: A COMPARATIVE STUDY BY MEANS OF MICROSCOPY AND MULTIPLEX PCR. Journal of Medical Genetics and Clinical Biology, 1(9), 60–71. https://doi.org/10.61796/jmgcb.v1i9.940
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References

  1. . N. Dogan, "Intestinal Parasites from Past to Present: Taxonomy, Paleoparasitology, Geographic Distribution, Prevention and Control Strategies," 2024, doi: 10.5772/intechopen.1005750.
  2. . A. Al-Hadheq, M. Al-Eryani, W. Edrees, and T. Al-Nosary, "Prevalence of Intestinal Parasitic Infections Among Children Attending Some Schools in Amran Governorate, Yemen," 2023, doi: 10.21203/rs.3.rs-3355603/v1.
  3. . K. Deepika, A. Baliyan, A. Chaudhary, and B. Sharma, "Advancement in the Identification of Parasites and Obstacles in the Treatment of Intestinal Parasitic Infections: A Brief Overview," 2024, doi: 10.5772/intechopen.1005455.
  4. . R. L. Richard and H. Yusof, "Advancements in Parasite Diagnosis and Challenges in the Management of Parasitic Infections: A Mini Review," Regional Conference on Science, Technology and Social Sciences (RCSTSS 2016), pp. 667-677, Mar. 2018, doi: 10.1007/978-981-13-0074-5_64.
  5. . Q. Liu, X. Jin, J. Cheng, H. Zhou, Y. Zhang, and Y. Dai, "Advances in the Application of Molecular Diagnostic Techniques for the Detection of Infectious Disease Pathogens (Review)," Mol. Med. Rep., vol. 27, no. 5, p. 104, May 2023, doi: 10.3892/mmr.2023.12991.
  6. . K. Algrooni, H. Alhaidan, H. Hamzah, M. Almarzouqi, and A. Hamad, "Innovative Approaches to Microbial Identification Enhancing Accuracy and Speed in Infectious Disease Diagnostics," Int. J. Community Med. Public Health, vol. 11, pp. 3231-3234, 2024, doi: 10.18203/2394-6040.ijcmph20241966.
  7. . H. Hasan, I. Rasheed, and N. Ahmed, "Molecular Epidemiological Characterization of Giardia Lamblia in Kirkuk, Iraq," Int. J. Multidiscip. Res. Anal., vol. 7, 2024, doi: 10.47191/ijmra/v7-i07-22.
  8. . H. K. Hasan, A. B. Mohammed, and W. M. S. Mero, "Detection and Molecular Identification of Entamoeba Species in Fecal Samples from Duhok Province, Kurdistan Region, Iraq," Ann. Parasitol., vol. 70, no. 2, pp. 91-101, 2024, doi: 10.17420/ap7002.526.
  9. . M. Shakir, "Assessment of Waterborne Parasites in Drinking Water Sources in Iraq," Int. J. Adv. Res., vol. 12, pp. 95-106, 2024, doi: 10.21474/IJAR01/18097.
  10. . "Intestinal Parasitic Infection Among Children Less Than Five Years of Age Visiting Children’s Hospital of Kathmandu," J. Inst. Med. Nepal, vol. 40, no. 2, pp. 78-83, Jul. 2024. [Online]. Available: https://jiomnepal.edu.np/index.php/jiomnepal/article/view/856. Accessed: Sep. 22, 2024.
  11. . F. Shan, A. Khan, M. Khan, and A. Rahat, "Detecting Intestinal Parasites in Diarrheal Cases Among Children in Dir Lower District," J. Health Rehabil. Res., vol. 4, pp. 1281-1285, 2024, doi: 10.61919/jhrr.v4i1.537.
  12. . A. N. M. Gubran, N. M. Al-Haidary, M. F. M. Bajubair, A. M. A. Algibary, M. G. M. Ali, and M. F. O. Ali, "Intestinal Parasites Among Primary School Children in Aden, Yemen," 2024, doi: https://doi.org/10.1101/2024.08.12.24311851.
  13. . R. Agrawal, S. Pattnaik, J. S. Kshatri, S. Kanungo, N. Mandal, S. K. Palo, and S. Pati, "Prevalence and Correlates of Soil-Transmitted Helminths in Schoolchildren Aged 5 to 18 Years in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis," Front. Public Health, 2024, doi: 10.3389/fpubh.2024.1283054.
  14. . G. Ahmed, N. M. Al-Haidary, M. F. M. Bajubair, A. M. A. Algibary, and M. A. Ali, "Intestinal Parasites Among Primary School Children in Aden, Yemen," 2024, doi: 10.1101/2024.08.12.24311851.
  15. . S. Shrestha, S. Raya, L. Shrestha, K. Parajuli, and J. B. Sherchand, "Intestinal Parasitic Infection Among Children Less Than Five Years of Age Visiting Children’s Hospital of Kathmandu," 2024, doi: 10.59779/jiomnepal.856.
  16. . R. Agrawal, S. Pattnaik, J. S. Kshatri, S. Kanungo, N. Mandal, S. K. Palo, and S. Pati, "Prevalence and Correlates of Soil-Transmitted Helminths in Schoolchildren Aged 5 to 18 Years in Low- and Middle-Income Countries: A Systematic Review and Meta-Analysis," Front. Public Health, 2024, doi: 10.3389/fpubh.2024.1283054.
  17. . D. Gebretsadik, Y. Metaferia, A. Seid, G. M. Fenta, and A. Gedefie, "Prevalence of Intestinal Parasitic Infection Among Children Under 5 Years of Age at Dessie Referral Hospital: Cross Sectional Study," BMC Res. Notes, vol. 11, no. 1, p. 771, Oct. 2018, doi: 10.1186/s13104-018-3888-2.
  18. . C. Scholz, V. H. Jarquín-Díaz, A. Planillo, V. Radchuk, C. Scherer, C. Schulze, S. Ortmann, S. Kramer-Schadt, and E. Heitlinger, "Host Weight, Seasonality, and Anthropogenic Factors Contribute to Parasite Community Differences Between Urban and Rural Foxes," Sci. Total Environ., 2024, doi: 10.1016/j.scitotenv.2024.173355.
  19. . C. Scholz, V. H. Jarquín-Díaz, A. Planillo, V. Radchuk, C. Scherer, C. Schulze, S. Ortmann, S. Kramer-Schadt, and E. Heitlinger, "Host Condition, Seasonality and Environmental Factors Explain Parasite Community Differences Between Urban and Rural Foxes," 2023, doi: 10.22541/au.167819105.52622439/v1.
  20. . V. Pankao, P. Chantree, and P. Martviset, "Global Warming and Parasitic Infection in Urban Communities: A Systematic Review," Vajira Med. J., 2024, doi: 10.62691/vmj.2024.267469.
  21. . L. D. Alenou, P. Nwane, L. R. Mbakop, M. Piameu, W. Ekoko, S. Mandeng, E. N. Bikoy, J. C. Toto, H. Onguina, and J. Etang, "Burden of Mosquito-Borne Diseases Across Rural Versus Urban Areas in Cameroon Between 2002 and 2021: Prospective for Community-Oriented Vector Management Approaches," Parasites Vectors, vol. 16, no. 1, p. 136, Apr. 2023, doi: 10.1186/s13071-023-05737-w.
  22. . E. Ahmed, S. K. Karim, A. A. Raza, and P. Siddiqua, "Prevalence and Risk Factors Associated with Human Intestinal Parasitic Infections (IPIs) in Rural and Urban Areas of Quetta, Pakistan," Braz. J. Biol., 2023, doi: 10.1590/1519-6984.266898.
  23. . S. Sharifah, S. Syed, M. Baharom, S. M. Amir, N. Bahari, M. R. Hassan, S. S. A. Rahim, M. S. Jeffree, A. R. Ramdzan, A. Atil, K. Mokti, M. F. Madrim, M. A. A. Rahim, Z. N. S. Ahmad, "Helminth Infection Among Children Living in an Urban Area in Tropical Countries: A Systematic Review," Open Access Macedonian Journal of Medical Sciences, 2023, doi: 10.3889/oamjms.2023.11176.
  24. . M. D. N. Ayubi, A. K. Raut, M. D. K. Rashid, and S. Chandra, "Prevalence and Associated Risk Factors of Human Intestinal Parasitic Infections in Rural Areas," International Journal of Health Sciences [Online], vol. 6, no. S7, pp. 5542-5550, Oct. 2022. Available: https://sciencescholar.us/journal/index.php/ijhs/article/view/13279. [Accessed: Sept. 22, 2024].
  25. . C. Scholz, V. Jarquín-Díaz, A. Planillo, V. Radchuk, C. Scherer, C. Schulze, S. Ortmann, S. Kramer-Schadt, and E. Heitlinger, "Host Condition, Seasonality and Environmental Factors Explain Parasite Community Differences Between Urban and Rural Foxes," 2023, doi: 10.22541/au.167819105.52622439/v1.
  26. . T. Sema, Ö. K. Özyurt, G. Öngüt, H. Yazisiz, F. Ö. Eryiğit, B. Özhak, L. Dönmez, A. O. Sekercioglu, and D. Ögünç, "Evaluation of the Methods Used for the Detection of Entamoeba histolytica in Stool Samples of Patients with Diarrhea," Mikrobiyoloji Bulteni, 2022, doi: 10.5578/mb.20229606.
  27. . A. K. A. R. Al-Tamemy and Z. Ahmed, "The Molecular Investigation of Entamoeba histolytica by Nested Multiplex PCR in Diarrheogenic Patients," International Journal of Health Sciences [Online], vol. 6, no. S6, pp. 489-498, Jun. 2022. Available: https://sciencescholar.us/journal/index.php/ijhs/article/view/9635. [Accessed: Sept. 22, 2024].
  28. . A. Dashti, H. Alonso, C. Escolar-Miñana, P. C. Köster, B. Bailo, D. Carmena, and D. González-Barrio, "Evaluation of the Use of Singleplex and Duplex CerTest VIASURE Real-Time PCR Assays to Detect Common Intestinal Protist Parasites," Diagnostics, vol. 14, no. 3, p. 319, Feb. 2024, doi: 10.3390/diagnostics14030319.
  29. . A. Bin Aziz, N. Roshidi, M. Hanif, G. Tye, and N. Arifin, "Giardia Lamblia Immunoassay: Systematic Review and Meta-Analysis," Clinica Chimica Acta, vol. 561, p. 119839, 2024, doi: 10.1016/j.cca.2024.119839.
  30. . S. Gabrielli, G. L. Milardi, L. Scarinci, C. Fanì, and M. Trotta, "Comparative Performance Evaluation of Four Different Methods for Diagnosing Giardia Infection in Dogs and Zoonotic Assemblages' Identification," Veterinary Parasitology, vol. 329, p. 110192, Jul. 2024, doi: 10.1016/j.vetpar.2024.110192.
  31. . S. Muhsin and I. Daoud, "A Comparison Between Microscopic and ELISA Techniques in the Diagnosis of Giardia lamblia Infection with Epidemiological and Biochemical Study Among Children Under Ten Years in Tikrit District," Tikrit Journal of Pure Science, vol. 20, pp. 65-69, 2023, doi: 10.25130/tjps.v20i3.1188.
  32. . W. Felefel, A. Abdel-Rady, I. Abd El-Rahim, M. Elkamshishi, and W. Mostafa, "Detection of Cryptosporidium parvum in Calf Feces Using Microscopical, Serological, and Molecular Methods," Iraqi Journal of Veterinary Sciences, vol. 37, pp. 383-389, 2023, doi: 10.33899/ijvs.2022.134661.2390.
  33. . M. Katiyar, S. Padukone, R. Gulati, and R. Singh, "A Multiplex PCR Assay for the Detection of Cryptosporidium Species and Simultaneous Differentiation of Cryptosporidium hominis and Cryptosporidium parvum in Clinical Stool Samples," 2023, doi: 10.1101/2023.03.22.533796.
  34. . G. Robinson, K. Elwin, M. Jones, and R. M. Chalmers, "A Comparison of qPCR and Microscopy for the Detection and Enumeration of Cryptosporidium Oocysts from Drinking Water," Journal of Medical Microbiology, vol. 72, no. 6, 2023, doi: 10.1099/jmm.0.001715.
  35. . E. M. Gonçalves, R. S. Araújo, M. Orban, G. R. Matté, M. H. Matté, and C. E. Corbett, "Protocol for DNA Extraction of Cryptosporidium spp. Oocysts in Fecal Samples," Revista do Instituto de Medicina Tropical de São Paulo, vol. 50, no. 3, pp. 165-167, May 2008, doi: 10.1590/s0036-46652008005000002.
  36. . S. Mormeneo Bayo, E. López González, A. Bellés Bellés, A. Bernet Sánchez, J. Aramburu Arnuelos, I. Jiménez Pérez de Tudela, I. Prats Sánchez, and M. García González, "Detection and Pathological Role of Intestinal Protozoa in Children," Parasitology International, vol. 88, p. 102558, Jun. 2022, doi: 10.1016/j.parint.2022.102558.
  37. . A. Nicolas, C. Nourrisson, A. Abou-Bacar, P. Poirier, S. Valot, A. Laude, G. Desoubeaux, C. Pomares, M. Machouart, Y. Le Govic, F. Selle, F. Botterel, N. Bourgeois, E. Cateau, M. Leterrier, P. Le Pape, F. Morio, and S. Houzé, "Selecting a Multiplex PCR Panel for Accurate Molecular Diagnosis of Intestinal Protists: A Comparative Study of Allplex® (Seegene®), G-DiaParaTrio (Diagenode®), and RIDA®GENE (R-Biopharm®) Assays and Microscopic Examination," Parasite, 2022, doi: 10.1051/parasite/2022003.