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Abstract
Elected(40) a random sample of raisin juice from both sides of Mosul city (right and left) and its suburbs, which included the areas of Bashiqa, Al-Hamdaniya, Bartella, Al-Shekhan, Aqra and Dohuk, for the period from January 2023 to April 2024, to detect the extent of contamination of these samples with fungi and yeasts using the culture media Potato Dextrose Agar and Sabouraud Sucrose Agar. The results of the culture of the samples showed that there were fungi that grew on the two media and others that grew on one media. All isolates were diagnosed based on their morphological and biochemical characteristics and diagnosis using the planting medium. (121) fungal isolates of different species were obtained with a contamination rate of (100%) distributed as follows: The highest contamination rate for fungal species 33/121 (27.3%). Candida spp. and (27.3%)33/121 Apergillus spp., respectively, while I reached Contamination rate of raisin juice with Wickerhamomycesanomalus15/121(12.39%),Saccaromyces cervisiae14/121(11.57%), Rhotodorula spp.14/121(8.26%), and Penicillium ssp. and Exophiala dermatitidis (3.3%) each. Four fungal isolates wereFor each of them, followed by the following fungal species: Geotrichum spp. and Fusarium spp. and Epidermophytes floccosum by percentage pollution reached(0.82%) respectively but Pollution rate by Mushrooms(4/121(2.47% Cladosporiumoxysporu and (1.65%)2/121 Trichophyton mentagrophytonThe results of the study showed that (26)A sample of raisin juice was heavily contaminated with filamentous fungi and yeasts.By (65%)And it isR1, R3, R4, R5, R7, R9, R10, R11, R12, R14, R15, R16, R17, R19, R20, R21, R22, R26, R28, R29, R30, R35, R36, R38, R39, and R40. The other samples were contaminated with fungi at a low rate. And I ranto be sureDiagnosisfor fungal isolationUsing molecular methods to compare nitrogenous base sequences at the National Center for Biotechnology InformationNCBI using BLAST And it was recordedstrainnewAndCandida oleophilastrain MAYKAIY and sequenced as PP961930.1 in GenBankGlobal.
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References
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References
. M. Mandappa, K. Basavaraj, and H. K. Manonmani, "Analysis of Mycotoxins in Fruit Juices," in Fruit Juices, Wageningen, The Netherlands: Academic Press, 2018, pp. 763–777.
. L. Kui, M. Tang, S. Duan, S. Wang, and X. Dong, "Identification of Selective Sweeps in the Domesticated Table and Wine Grape (Vitis vinifera L.)," Front. Plant Sci., vol. 11, p. 572, 2020. doi: 10.3389/fpls.2020.00572.
. N. Anjum, M. A. Feroze, R. Rafique, and M. H. Shah, "Effect of Gibberellic Acid on Berry Yield and Quality Attributes of Grapes cv. Sultanina," Pure Appl. Biol. (PAB), vol. 9, no. 2, pp. 1319–1324, 2020. doi: 10.19045/bspab.2020.90137.
. P. K. Jaiswal, R. Kesharwani, D. K. Patel, P. Verma, and V. Kumar, "Nutraceuticals in Agriculture," in Nutraceuticals, Academic Press, 2023, pp. 223–239.
. R. Khiari, H. Zemni, and D. Mihoubi, "Raisin Processing: Physicochemical, Nutritional, and Microbiological Quality Characteristics as Affected by Drying Process," Food Rev. Int., pp. 1–53, 2018. doi: 10.1080/87559129.2018.1517264.
. C. De Torres, R. Schumacher, M. E. Alañón, M. S. Pérez-Coello, and M. C. Díaz-Maroto, "Freeze-Dried Grape Skins By-Products to Enhance the Quality of White Wines from Neutral Grape Varieties," Food Res. Int., vol. 69, pp. 97–105, 2015. doi: 10.1016/j.foodres.2014.12.016.
. D. Wang, J. Cai, B. Q. Zhu, G. F. Wu, C.-Q. Duan, G. Chen, and Y. Shi, "Study of Free and Glycosidically Bound Volatile Compounds in Air-Dried Raisins from Three Seedless Grape Varieties Using HS-SPME with GC-MS," Food Chem., vol. 177, pp. 346–353, 2015. doi: 10.1016/j.foodchem.2015.01.018.
. C. Venkitasamy, L. Zhao, R. Zhang, and Z. Pan, "Grapes," in Integrated Processing Technologies for Food and Agricultural By-Products, Z. Pan, R. Zhang, and S. Zicari, Eds., Academic Press, 2019, pp. 133–163. doi: 10.1016/B978-0-12-814138-0.00006-X.
. M. Imran, M. S. Arshad, M. S. Butt, J. H. Kwon, M. U. Arshad, and M. T. Sultan, "Mangiferin: A Natural Miracle Bioactive Compound Against Lifestyle-Related Disorders," Lipids Health Dis., vol. 16, pp. 1–17, 2017.
. U. N. Das, "Essential Fatty Acids and Their Metabolites Could Function as Endogenous HMG-CoA Reductase and ACE Enzyme Inhibitors, Anti-Arrhythmic, Anti-Hypertensive, Anti-Atherosclerotic, Anti-Inflammatory, Cytoprotective, and Cardioprotective Molecules," Lipids Health Dis., vol. 7, pp. 1–18, 2008.
. P. Restani, G. Frigerio, F. Colombo, L. P. de Sousa, A. Altindişli, R. F. Pastor, and C. Di Lorenzo, "Raisins in Human Health: A Review," in BIO Web Conf., vol. 7, p. 04005, 2016.
. J. E. Welke, "Fungal and Mycotoxin Problems in Grape Juice and Wine Industries," Curr. Opin. Food Sci., vol. 29, pp. 7–13, 2019.
. Pena, F. Cerejo, L. J. G. Silva, and C. M. Lino, "Ochratoxin A Survey in Portuguese Wine by LC-FD with Direct Injection," Talanta, vol. 82, pp. 1556–1561, 2010.
. L. Freire, F. R. F. Passamani, A. B. Thomas, L. M. Silva, F. N. Paschoal, G. E. Pereira, G. Prado, and L. R. Batista, "Influence of Physical and Chemical Characteristics of Wine Grapes on the Incidence of Penicillium and Aspergillus Fungi in Grapes and Ochratoxin A in Wines," Int. J. Food Microbiol., vol. 241, pp. 181–190, 2017.
. M. A. Ronald, E. B. AIFard, and C. P. Lawrence, Laboratory Manual of Experimental Microbiology, U.S.A: Mosby Year Book, Inc., 1995, pp. 212–220.
. E. H. Marth, Standards Methods for the Examination of Dairy Products, 14th ed., Washington, DC: American Public Health Association, 1978, p. 416.
. G. S. de-Hoog and J. Guarro, Atlas of Clinical Fungi, Spain: Universitat Rovira i Virgili, 1995, p. 720.
. J. I. Pitt and A. D. Hocking, "Methods for Isolation, Enumeration, and Identification," in Fungi and Food Spoilage, 3rd ed., Boston, MA: Springer, 1997. doi: 10.1007/978-1-4615-6391-4_4.
. R. A. Samson, E. S. Hoekstra, and J. C. Frisvad, Introduction to Food and Airborne Fungi, 7th ed., Utrecht, The Netherlands: Centraalbureau voor Schimmelcultures, 2004.
. Alomari, A. Bahkali, A. Asran, and A. Elgorban, "Toxigenic Fungi Isolated from Dried Fruits: Detection of Aspergillus Toxins in Saudi Arabia," J. Pure Appl. Microbiol., vol. 8, no. 1, 2014.
. T. Iamanaka, M. H. Taniwaki, H. C. Menezes, E. Vicente, and M. H. P. Fungaro, "Incidence of Toxigenic Fungi and Ochratoxin A in Dried Fruits Sold in Brazil," Food Addit. Contam., vol. 22, no. 12, pp. 1258–1263, 2005.
. A. E. Allam, A. E. E. Tantawy, K. A. E. Mohamed, E. A. Morad, and M. A. E. El Shafei, "Otitis Externa in a Tertiary Care Hospital in Zagazig, Egypt: Isolated Pathogens and Their Antibiotic Sensitivity Patterns," Afr. J. Clin. Exp. Microbiol., vol. 21, no. 1, pp. 60–65, 2020.